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ea0035oc12.3 | Pituitary Basic | ECE2014

Detection of pituitary antibodies by immunofluorescence: Methodological approach and results in patients with pituitary diseases

Ricciuti Adriana , De Remigis Alessandra , Landek-Salgado Melissa A. , De Vincentiis Ludovica , Guaraldi Federica , Lupi Isabella , Iwama Shintaro , Wand Gary S. , Salvatori Roberto , Caturegli Patrizio

Pituitary antibodies have been measured mainly to identify patients whose disease is caused or sustained by pituitary-specific autoimmunity. Although reported in over 100 publications, this antibody test has variable results and limited clinical utility. Our goals were to: identify major sources of variability; test them experimentally for establishing an optimized immunofluorescence protocol; assess prevalence and significance of pituitary antibodies in patients with pituitar...